Antifungal Effect of Liposomal ?-Bisabolol and When Associated with Fluconazole

Fungal pathologies caused by the genus Candida have increased in recent years due to involvement of immunosuppressed people and advance resistance mechanisms acquired these microorganisms. Liposomes are nanovesicles with lipid bilayers which they store compounds. ?-Bisabolol is a sesquiterpene proven biological activities, this work it was tested alone liposomes association Fluconazole vitro evaluate antifungal potential, optimization, virulence inhibitory effect vitro. Antifungal assays were performed against standard strains albicans, tropicalis, krusei microdilution identify IC50 values obtain cell viability. The Minimum Fungicidal Concentration (MFC) subculturing on solid medium, at their subinhibitory concentration (Matrix (MC): 16,384 µg/mL) (MC/16), compounds, both isolated liposomal, associated fluconazole order verify junction. Tests ascertain changes morphology microculture chambers according MC concentrations. characterized from vesicle size, polydispersity index, average Zeta scanning electron microscopy. value liposomal bisabolol (FCZ) 2.5 µg/mL all tested, revealing potentiating effect. Liposomal able potentiate CA CT reducing its completely inhibiting fungal growth. form inhibited morphological transition MC/8. homogeneous, vesicles diameters 203.8 nm for surface charge potential ?34.2 mV, conferring stability nanosystem. Through microscopy, spherical shapes observed.